When we got our 2nd Golden puppy Sammy, I noticed that his eyes were very dark brown compared to our 3 year old Kasey's eyes, (pictured below) which are fairly light in color. I just thought it was a puppy thing and Sammy's eyes would get lighter in color as he grew up. He is now about 9 months old and his eyes are still really dark. So my question is, are there different colored eyes in Goldens, like we humans have?

Yes, there are varying eye colors that are acceptable. You need to look up the standard on the AKC website or the GRCA website to see what eye color should be...

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Janice and The Celebration Gang -
"Samantha, George, Tiki, Emily, Mick and Basil" Gone but not forgotten, Sally(Windjammer's Ima Country Girl CDX CGC), Laney(Mandell Marlenes Celebration UD RA CGC), and Cookie(Starseeker's Kissmas Cookie CDX RE CGC).

Here is a direct quote from the GRCA standard:
Color preferably dark brown; medium brown acceptable.

The fact that it specifically mentions dark and medium suggests that there were eye color variations when it was written.

All the goldens I have personally seen have had dark eyes. Though, I have heard folks mention goldens with lighter eyes.

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Laura, Jinx, and Rocky
UKC CH Wildfire's A Kind of Magic CGC
http://www.k9data.com/pedigree.asp?ID=474956
Rocky the old min pin who thinks he's a golden

The color does vary. I think Kasey's (in the picture) are pretty light and the color ranges to very dark. As I recall, the Golden breed standard mentions eye color. I think it says dark is preferred but medium brown is acceptable. Our Zeke's eyes were a medium brown and I thought they were somewhat more expressive than the very dark eyes of some Goldens.

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Zoe, Rockwall Nantucket Breeze, BN, CGC, Delta therapy dog
Zeke, our introduction to the world of Golden Retrievers

Axl's eyes are light to medium brown. Angus, my 8 month old, 80 lb 'puppy' has very dark brown eyes.

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Axl doing what he was BORN to do...

Sadie's are really dark. in most lights I can't differentiate between the pupil and the iris.

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Loving My Girl Sadie

"I think dogs are the most amazing creatures; they give unconditional love. For me they are the role model for being alive."
- Gilda Radner